About a month ago one of my coworkers, Aaron Lum Ph.D and I, had a chance to compare various light sources and to measure the power they provide. Most importantly, we were able to compare LED engines against other conventional light sources. As a followup, Aaron was able to work with Logan Grosenick from Stanford (Thanks Logan!) , to compare the Spectra light engine against the Sutter Instruments DG-5. The results are graphed below. The vertical axis is in mW.
My conclusions to the data are fundamentally unchanged from the previous report: LED’s stink at producing short wave UV. The real market application for the DG-5 continues to be calcium ratio dyes like FURA. As of now, no LED source can compete at anywhere the power level needed for FURA imaging (at 340 & 380nm lines) like the DG-5 can. I expect, as with numerous other examples of disruptive technology, this will change over the next few years. LED powered systems should be considered for all other applications.
Data provided by Logan Grosenick, PhD.-Austin

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