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Overview: Traditional transmitted lamps used on microscopes have several limitations. They don’t auto-shutter (turning off and on is rather slow, and requires the addition of a mechanical shutter for high speed multi-site acquisition), they change color spectrum as brightness is increased or decreased, and they have a rather short lifetime. To top it off, they…

Sorry to be away for so long! Still working on a special project which I will illuminate soon… In the meantime. I’ve collected up my favorite apps for microscopy: #1. Rescalc – I consider this #1 because of it’s immediate and useful application for our specific field. Nothing but light microscopy here. From resolution estimates…

This is good news! Sorry posting’s been light. I’ve been working on a project which I will post about quite a bit in the coming weeks. But for now – it’s a secret 🙂 -Austin    

This is a nice alternative to a $7,000 spectrophotometer. It’s also a lot smaller and hackable!  

Andrew York from the NIH has done this, and is reporting good results thus far. Interesting technique! I’m curious how the overall sensitivity of the instrument is, but the examples posted thus far look great! PDF  – Google Code Site – Nature Methods    

Yesterday evening I was able to collect a timelapse series of the annular eclipse. My location was outside of the complete annulus, but it was quite close! Below is a quick youtube video of the timlapse as well as a few stills. I used a Nikon D80 with a DX sensor, coupled to a 80-300mm…

This post will grow over time, but I’m posting this so that researchers and vendors have access to one single repository of filter info. Hopefully this will make all of our lives easier! Definitions: ET – Chroma name for a sputter coated filter. Multiband – filter set which allows multiple channels to pass through it…

I must apologize for my lack of posts – I’ve been traveling a lot these past few weeks for work. In my travels I’ve come across this exceptional timelapse montage from Shawn Reeder. He spent two years putting the shots together. Timelapse work is extremely difficult, and it takes a big investment on the photographer…

One of the most overlooked capabilities possessed by digital capture is what can be accomplished by image processing after capture. In modern EMCCD cameras, some processing is done directly in the camera head. Simple adjustments like background correction or shading correction are well known, but more complex operations, like HDR , or High Dynamic Range…

I had a customer recently ask for these, and I thought I’d post them. These are from Nikon Inc. I’ll also have them on the resources page for reference. These include Useful magnification, Brightness Factor, Refractive Index, Depth of Field at Image Plane, Depth of Focus at Specimen Plane, Useful Magnification and Infinity Optics Formulas.…