Category: uManager
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Cooling a Pt Grey Camera
I’ve been working with Pt Grey (now FLIR) cameras for a few years now, and one nagging question in the back of my mind has been whether or not it is possible to cool them via external means. There are basically 3 categories of cooling when it comes to low light cameras: no cooling whatsoever…
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Automated Capture and Save script for Micro-Manager
I wrote this for a client, but figured others could make use of it. This code simply captures a number of images as defined by the user, and saves them to the specified folder without displaying them. This is useful if you don’t want all of the images open in memory during a timelapse. Simple…
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Triggerscope & Micro-Manager Demo LED
With ASCB right around the corner, I’ve been looking for opportunities to showcase the controller, so I’m happy and grateful to announce that the TriggerScope16 will be demonstrated at the Open-Imaging Micro-Manager booth (#1312) this year! Here’s a short video I made showing the new display which will be at the booth. The display uses…
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Acquisition Speed Comparison of MetaMorph, NIS Elements, Micro-Manager
How fast can a typical imaging system really run? What factors affect this? While most research might not require rapid sequential capture, almost all experiments can benefiet from tight exposure/illumination timing, yet few microscope users are aware of timing delays injected into experiments by the control software they are using. So how much delay is…
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ImageJ Counting Macro for Known Field Values
Here’s a simple method to deal with varying intensities in a large run of images, assuming you have a known number of cells in a field. If I assume I should see, say, more than X cells per area, I can adjust my threshold to slowly increase it’s aggressiveness until that minimum counted value is…
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Acquisition crashes, and power settings in Win7
Windows 7 has a new power management interface, and the design of modern motherboards, along with win7 Automatic Power Management (APM), can cause some insidious device-based software crashes if power options aren’t set correctly. Here’s how to make sure your system is running in the best mode. Open the Windows Control panel Search for and…
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Determining background sources in fluorescence
For microscopy engineers and suppliers like me, the background produced in an image is of importance, if that background is created by the imaging system itself. What so many of us on this side of the scope (vs the user side) forget to train on and talk about is the specimen side of the background.…
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*UPDATED* Micro-Manager Tethered to a Nikon Digital SLR
***UPDATE 3/3/2014*** After a few requests for a report on x64 capability using this tethering technique, I tried installing the NK Remote software on a rather fresh copy of Windows 7×64 – it did not communicate with the camera at all. On the other hand, the open source application “DigiCamControl“, worked perfectly. Now, as far…