Thanks to everyone who attended my first course on image processing (and acquisition as it turns out). As promised here are some links:
– MBF Tools for ImageJ. This is a suite of processing and analysis tools, provided by the McMaster Biophotonics Facility, which can be used for, among other things, splitting apart pigment based IHC specimen images.
***One of the course attendees asked how to merge multiple channels, without using primary color (RGB) values. For instance one may want to select “Red, Cyan and Green”. This function is available using the MBF Tools function found under Plug-Ins, then under the “Colour Functions” and finally using the “Colour Merge” command.
– This tool only merges two channels. It is possible to run this multiple times, so a merge can have a third channel added to it, and so-on.
– LOCI Formats Importer/Exporter is a tool which can be used to read and write many file types. It can also import metadata from many of the proprietary file types used today.
– I was not able to find a dynamic and interactive particle counting plugin for ImageJ, however when you have produced a count by selecting “Analyze particles” you can select the “Distribution” command from the Analyze menu to produce histogram distributions for every measurement type selected.
Please contact me if you have any other items you’d like me to cover. Thanks again!
– Austin