I wrote this for a client, but figured others could make use of it. This code simply captures a number of images as defined by the user, and saves them to the specified folder without displaying them. This is useful if you don’t want all of the images open in memory during a timelapse.
Simple Capture and Save Beanshell Script
-Austin
Here’s a simple method to deal with varying intensities in a large run of images, assuming you have a known number of cells in a field. If I assume I should see, say, more than X cells per area, I can adjust my threshold to slowly increase it’s aggressiveness until that minimum counted value is met. The code can be downloaded as an imageJ macro here. but here’s a walk through of this simple script. My comments are preceded by a *.:
rnum = 0; *this creates a new variable, or container, for our # of regions
tval = 2000; *this is the starting object threshold value.
while (rnum<500) *this is a "while" loop. The loop runs until the argument is satisfied.
{
setAutoThreshold("Default");
setThreshold(tval, 4095); *note that tval = the minimum threshold value
run("Analyze Particles...", "size=0-Infinity circularity=0.00-1.00 show=Nothing display clear add");
rnum = roiManager("count");
tval = tval - 100; *tval continues to become more aggressive in counting objects until the argument is met.
}
Simple routines like these can reduce your headache factor by a large margin if you learn how to harness the power of macros!
-Austin
Yesterday we received our first shipment of Sola white light LED driven sources for microscopy. I had a chance to snag one and write a driver for it. Price is $300 USD. The driver controls both the shuttering and ND on the box, and integrates easily into Elements as a shutter and ND filter wheel.
Please contact me if you are interested!
-Austin
I’ve written a Lumencor Spectra Driver that works with NIS Elements B3.2+. (AR or BR + Advanced Interpreter) The driver provides integrated control into elements for both intensity of lines and channel. These appear as a shutter and two filter wheels, offering full integration into Elements’ Optical configurations. We are selling it for $750, including online install on your system. Please contact me if you’d like to try it out!
-Austin