I had a customer interested in some rather tough mitochondrial analysis. None of the tools he had available were working. After some homework we found that the kymograph tools provided by J. Rietdorf and A. Seitz included a full velocity output capability. The plugins are not provided in Fiji, so you’ll need to manually install them (download page here), which is actually quite straightforward. For an example of how these tools work check out this workflow document provided by the authors.
– Austin
J. Rietdorf and A. Seitz
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