Laura Sysko from Nikon sent us a How-To on outputting live graphs that can be included with a timelapse or other ND file. You can find the How To Here.
The SIM technique was developed by Dr. Mats Gustaffsen, Dr. John Sedat and Dr. David Agard at UCSF. The technology doubles the XY resolution of a traditional CLSM, using a structured illuminations technique and custom manufacturing process. This method also supports 3-D sectioning as well as TIRF.
The STORM system was developed by Dr. Xiaowei Zhuang at Harvard University. This system uses statistical sampling of multiple exposures to produce extremely high resolution images. It supports 3-D image construction as well.
At the show floor Nikon had the A1R-MP Confocal system running. This system can perform very high frame rate acquisitions using multiphoton excitation.
Key features are:
420 fps with MP excitation
Simple switching between resonant and non descanned detector systems. (done on the fly with no need to change hardware)
Can be coupled with Plan Apo Water Immersion IR 60x lens
Hamamatsu has released a dual chip camera for ratiometric applications. The camera includes 2 of the ER-150 CCD’s, with one chip supporting discreet focusing, which allows the system to be chromatically aligned. Key features of this system are:
Electronically linked filter cubes that auto-configure the camera for use.
Independent focusing between CCD’s, X/Y translation is automatically performed by the camera.
Andor had it’s new ICX-285-based Clara camera and revolution DSD Confocal on display. Key features of the Clara camera are:
1Mhz readout mode for very low read noise imaging
Deep cooling capability at -45c
Simple USB interface
The Revolution DSD confocal is a new white light system that uses a transmission and reflection pattern to produce a confocal image. Andor has a webinar available on the DSD here.
Prior has released a new Proscan 3 controller. This includes a new joystick which has some nice programming of it’s wheel functions. The controller supports TTL, Filter wheels, Shutters and XYZ encoded motors.
Stopped by the sutter booth to check out the XL. It’s been out for a while but it’s always good to see. They had it running in a filter wheel loop for the duration of the show.