Files & Info

Presentations

Selecting the Best Camera

The camera is one of the highest cost items on a microscopy system, it also can have a large impact on the quality of your data collection. I’ve made a general guide to help get people started. As with all microscopy products it’s important to consult fellow researchers, imaging core administrators and local sales reps to determine your specific needs.  Click Here for the guide.

My “Camera architecture & Microscopy” Presentation

If you’ve attended one of my training courses on-site you probably saw this presentation used in referral to the design and general abilities of a modern scientific grade CCD camera. I’ve included a download here for review. If you are researching what camera to purchase you may want to review this, as it describes each major design used today.

My “Quantitative Imaging Fundamentals” Presentation

***This is a 90mb zip file***

One of the most common presentations I use in my training courses. This covers camera readout, image signal to noise, camera noise sources and the overview of basic image acquisition.

My “Image Analysis and Processing” Presentation

This is the course material for my presentation on image processing and analysis, primarily centered on using the freeware application ImageJ.

FAQ’s and Guides

Nikon Intensilight FAQ’s

Here’s a guide to everything you wanted or didn’t want to know regarding the Nikon Intensilight system. Click Here for the PDF. Thanks to Nikon’s Ed Lieser for putting this together!

How To’s

How to Merge Multiple Channels in Elements D (can also be used for BR and AR)

How to Save an Image for Display or Presentation

Calculated Objective Calibrations in Elements

Co-localization using Binary Thresholding

Using the Elements FRET Module

How to test COMM port on a PC

Saving ROI and Threshold overlays to a display image

Whole Field Analysis using Elements

Overlay Image Information in Image Pro Plus

TTL Device Control with Elements

Nikon Elements Downloads

Use this link to download the most current release of Nikon Elements software.

If you want to use a parallel port with Elements you’ll need the Parallel Port Macro (for v3.1)

Batch Export Macro – Use to export batches of ND files. ***for 32 Bit OS Only!

Grid to ND generator – Use to generate multipoint experiments from a user defined grid.

Well Plate Macro for Elements 3.1

Micropoint Driver – For 32 Bit OS Only

Mosaic 2 Driver – For 32 Bit OS Only

Elements 3.1 Installation Fix - This fixes the “App fails to launch” error found on 3.1 installs.

NIS Elements Free Viewer 3.1 – Used to view nd2 and other files. ***32 Bit operating systems ONLY

Lambda XL Start / Stop Driver – Use to control the start or stop of the Lambda XL light source from Elements.

PIOSetup.zip – This enables access to Elements parallel port functions – it also includes a sample setup macro from Nikon.

Miscellaneous Utilities for Device Control & Test

Parmon – This is a parallel port monitor and control tool.  It’s easier to use than a multimeter!

Free Serial Port Monitor – Use this to see if your programs are communiating correcly with your devices.

Startup Control Panel – Use this to enable or disable boot processes which can cause problems with imaging software. ***Be careful with this as you can inadvertently turn off all sorts of important processes if you don’t know what you are doing.

SyncBack – The freeware version is an excellent backup utility for backing up experimental data. Thanks to Del from UNR for introducing me to this great utility!