FRET Calculations in Elements 3.0

This summary demonstrates how to calculate FRET crosstalk between samples and how to apply these corrections to a recently acquired FRET sample.

Calculation

1. Calibrate FRET Images using samples loaded ONLY with Donor and then ONLY with Acceptor.

2. Open the “Create FRET Image” Window:

3. Load Sequences of acquired data (can be single images, time-lapse image sets or 3-D image stacks).

3: View FRET Image

4. Example of image sequence with dropping FRET efficiency. Change in FRET is graphed using Time Measurement: